Ashby’s Glucose Agar

ARTICLE NO. OLFC-250
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For the cultivation of Azotobacter species that can utilize mannitol as carbon and atmospheric nitrogen as nitrogen source.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Off white colored powder
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Milky white colored solution with stealing at bottom on standing
pH 7.4 ±0.2 at 25℃ (40.70 g/ l)
Solidification 40˚C
Color Milky white colored opalescent gel with slight precipitate

Contents

Ingredients Gram/Litre
Glucose20.00
Dipotassium Phosphate0.20
Magnesium Sulphate0.20
Sodium Chloride0.20
Potassium Sulphate0.10
Calcium Carbonate5.00
Agar15.00
pH at 25˚C7.4 ±0.2


Directions

Dissolve 40.70 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute in desire. Ensure complete solidification and inoculate test sample aseptically.