An ideal medium used for validation of aseptic packing and filling lines.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige to pink colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Orange red colored opalescent
pH
7.2 ±0.2 at 25℃ (17.50 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
5.000
Yeast Extract
2.500
Sucrose
5.000
Sodium Chloride
5.000
Phenol Red
0.005
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 17.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute aseptically. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15
min, cool it to 42-45 °C and inoculate test sample aseptically.
