For microbiological assay of Dihydrostreptomycin and Vancomycin by preparing inoculum
of Bacillus subtilis ATCC 6633
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent
pH
6.6 ±0.1 at 25˚C (30.80 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
6.000
Casein peptone
4.000
Yeast Extract
3.000
Meat Extract
1.500
Dextrose
1.000
Manganese sulphate
0.300
Agar
15.000
pH at 25˚C (after sterilization)
6.6 ±0.1
Directions
Dissolve 30.80 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C,
and distribute aseptically in sterile petri plates. Ensure complete solidification and inoculate test
organisms aseptically.
