For microbiological assay of Amphotericin B, Natamycin and Nystatin using
Saccharomyces Cerevisiae.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent
pH
6.1 ±0.1 at 25 ˚C (60.00 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
9.400
Yeast Extract
4.700
Meat Extract
2.400
Sodium Chloride
10.000
Dextrose
10.000
Agar
23.500
pH at 25˚C
6.1 ±0.1
Directions
Dissolve 60.0 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C,
and distribute aseptically in sterile petri plates. Ensure complete solidification and inoculate test
organisms aseptically.
