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Antibiotic Assay Medium No. 10 (Polymyxin Seed Agar)

ARTICLE NO. OLFC-205
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

As a seed layer medium for assaying the products containing Polymyxin-B, Carbenicillin, Colistin & Colistimethate sodium.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Light amber colored opalescent
pH 7.2 ±0.2 at 25˚C (42.00 g/ l) (10 ml/l polysorbate 80)
Solidification 40˚C
Color Light amber colored opalescent gel

Contents

Ingredients Gram/Litre
Pancreatic Digest of Casein17.000
Papaic Digest of Soybean3.000
Sodium Chloride5.000
Dibasic Potassium Phosphate2.500
Dextrose2.500
Agar12.000
pH at 25˚C7.2 ±0.2


Directions

Dissolve 42.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely add 10 ml of Polysorbate 80. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.