As a base agar for assaying the products containing Polymyxin-B.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent gel
pH
7.2 ±0.1 at 25 ˚C (50.00 g/ l)
Solidification
40˚C
Contents
Ingredients
Gram/Litre
Pancreatic Digest of Casein
17.000
Papaic Digest of Soybean
3.000
Sodium Chloride
5.000
Dibasic Potassium Phosphate
2.500
Dextrose
2.500
Agar
20.000
pH at 25˚C
7.2 ±0.1
Directions
Dissolve 50.0 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C,
and distribute aseptically (21 ml) in petri plates to form a base layer. Ensure complete
solidification and add 4 ml of seed layer of inoculum.
