Antibiotic Assay Medium No.2 (Base Agar)

ARTICLE NO. OLFC-185
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For microbiological assay of antibiotics.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Beige colored powder.
Solubility Soluble in Distilled/de-ionized water at 50°C
Color Light amber colored opalescent
pH 6.6 ±0.2 at 25 ˚C (25.50 g/ l)
Solidification 40 ˚C
Color Light amber colored opalescent gel

Contents

Ingredients Gram/Litre
Peptone6.000
Meat extract1.500
Yeast Extract3.000
Agar15.000
pH at 25˚C6.6 ±0.2


Directions

Dissolve 25.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and aseptically add Antibiotic assay medium No. 1 as seed agar, inoculated with the microbial culture.