For microbiological diffusion assay of several antibiotics in accordance with EP.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber colored opalescent
pH
6.55 ±0.05 at 25℃ (30.50 g/ l)
Solidification
40 ˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
6.000
Tryptone
4.000
Yeast Extract
3.000
Meat Extract
1.500
Dextrose
1.000
Agar
15.000
pH at 25˚C
6.55 ± 0.05
Directions
Dissolve 30.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
