As a basal medium to study fermentation reactions by adding carbohydrates.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Pinkish beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light pink colored clear solution without any precipitate.
pH
7.4 ±0.2 at 25˚C (15.00 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
10.000
Sodium Chloride
5.000
Andrade Indicator
0.100
pH at 25˚C
7.4±0.2
Directions
Dissolve 15.10 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically in test tubes containing inverted Durham’s tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to room temperature and aseptically add sterile stock solution of carbohydrate to a final concentration of 0.5% to 1.0% (w/v).
