For screening of anaerobes in cosmetics such as Talcum powder.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber, opalescent gel
pH
7.5 ±0.2 at 25˚C (50.41g/l)
Solidification
40 ˚C
Color
Light amber colored clear to slightly opalescent with fine precipitate.
Contents
Ingredients
Gram/Litre
Tryptone
15.000
Soya peptone
5.000
Yeast Extract
5.000
Sodium chloride
5.000
Hemin
0.05
Vitamin K1
0.010
L-Cystine
0.400
Agar
20.000
pH at 25˚C
7.5 ± 0.2
Directions
Dissolve 50.41 grams in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.