Medium to dark amber color, clear to slightly opalescent gel
pH
7.3±0.2 at 25℃ (40.55 g/ l)
Contents
Ingredients
Gram/Litre
Casein enzymic hydrolysate
17.000
Papaic digest of soyabean meal
3.000
Meat extract
7.500
Liver hydrolysate
3.000
Dextrose
6.000
Sodium chloride
2.500
Sodium thioglycollate
0.500
L-Cysteine
0.250
Sodium sulphite
0.100
Agar
0.700
pH at 25˚C
7.3±0.2
Directions
Dissolve 40.55 grams in 900 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45°C. Add 100 ml sterile serum and mix well. Dispense into a sterile test tube and inoculate the test sample aseptically.
