For cultivation of anaerobic bacteria especially Clostridium species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber colored opalescent medium
pH
7.2 ±0.2 at 25 ˚ C (58.00 g/ l)
Solidification
40 ˚C
Color
Light green color red slightly opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
20.000
Dextrose
10.000
Sodium chloride
5.000
Sodium thioglycolate
2.000
Sodium formaldehyde sulfoxylate
1.000
Methylene blue
0.002
Agar
20.000
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 58.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in test tubes or petri plates. Allow to solidify and inoculate test sample aseptically.
