For enrichment of potassium solubilizing bacteria from soil samples.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
A fine, free flowing, hygroscopic powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Off white colored solution with white precipitate
pH
7.2 ±0.2 at 25 ˚C (9.6 0 g/ l)
Solidification
40 ˚C
Contents
Ingredients
Gram/Litre
Magnesium Sulphate
0.500
Calcium Carbonate
0.100
Potassium Alumino Silicate
2.000
Ferric Chloride
0.500
Calcium Phosphate
2.000
Dextrose
5.000
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 9.60 grams in 1000 ml distilled water, check and adjust the pH if required so that after sterilization it is 7.2±0.2. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.