For the selective differentiation & isolation of Aeromonas hydrophila from clinical & environmental Specimens.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light yellow to pale cream-colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Pale yellow colored opalescent solution
pH
8.0 ±0.2 at 25℃ (56.30 g/ l)
Solidification
40 ˚C
Color
Light yellowish to creamy colored opalescent gel
Contents
Ingredients
Gram/Litre
Meat Peptone
5.000
Yeast extract
3.000
L-Lysine HCl
3.500
L-Arginine HCl
2.000
Inositol
2.500
Lactose
1.500
Sorbose
3.000
Xylose
3.750
Bile salts
3.000
Sodium thiosulphate
10.670
Sodium chloride
5.000
Ferric ammonium citrate
0.800
Bromo thymol blue
0.040
Thymol blue
0.040
Agar
12.500
pH at 25˚C
8.0 ±0.2
Directions
Dissolved 56.30 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically. DO NOT AUTOCLAVE. Cool it to 42-45 °C and add 5.0 mg Ampicillin for the selective isolation of Aeromonas. Mix well and aseptically pour in to the petri plates allow to solidify and inoculate test sample aseptically.
