For isolation and propagation of Actinomycetes from soil and water.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber colored opalescent solution
pH
8.1 ±0.2 at 25℃ (22.00 g/ l)
Solidification
40 ˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Sodium Caseinate
2.000
L-Asparagine
0.100
Sodium Propionate
4.000
Dipotassium Phosphate
0.500
Magnesium Sulphate
0.100
Ferrous Sulphate
0.001
Agar
15.000
pH at 25 ˚C
8.1 ±0.2
Directions
Dissolve 22.00 grams in 5% glycerol solution, boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
