For the detection of urease production by Proteus species and for identification of other
members of Enterobacteriaceae
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light orange red colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Yellow orange colored, clear opalescent
pH
6.8 ±0.2 at 25 ˚C (24.00 g/ l) (add 50 ml of sterile 40% Urea solution)
Solidification
40˚C
Contents
Ingredients
Gram/Litre
Peptone
1.000
Dextrose
1.000
Sodium Chloride
5.000
Monobasic Potassium Phosphate
2.000
Phenol Red
0.012
Agar
15.000
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 24.00 grams in 950 ml distilled water, boil to dissolve the medium completely
and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C and add 50
ml of sterile 40% Urea solution mix well and distribute in sterile test tubes. Slant the tubes to
have a small butt and slant, allow to solidify. Ensure complete solidification and inoculate test
sample aseptically.
