For identifying bacteria on the basis of urea utilization, for the differentiation of Proteus
species from Salmonella and Shigella species
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light orange pink colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Yellow orange colored, clear opalescent
pH
6.8 ±0.2 at 25˚C (18.70 g/ l)
Contents
Ingredients
Gram/Litre
Yeast Extract
0.100
Monobasic Potassium Phosphate
9.100
Dibasic Potassium Phosphate
9.500
Phenol Red
0.010
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 18.70 grams in 950 ml distilled water, boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure at 121˚C for 15 minutes. Allow to cool the
medium and aseptically add 50ml of sterile 40% urea solution. Mix well and distribute in sterile
test tubes and inoculate test sample aseptically.
