For differentiation of micro-organisms especially Enterobacteriaceae on the basis of their
ability to hydrolyze urea and indole production.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Tan colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light orange colored opalescent
pH
6.8 ±0.2 at 25 ˚C (30.00 g/ l)
Contents
Ingredients
Gram/Litre
L-Tryptophan
3.000
Sodium Chloride
5.000
Monobasic Potassium Phosphate
1.000
Dibasic Potassium Phosphate
1.000
Urea
20.000
Phenol Red
0.012
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 30.00 grams in 1000 ml distilled water. Mix gently till all the ingredients
dissolve completely. Filter sterilize the medium (DO NOT AUTOCLAVE) and distribute
aseptically in sterile test tube and inoculate test sample aseptically.
