For detection and enumeration of coliform bacteria in water and food.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light Beige to pink colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Reddish purple color, clear slightly opalescent
pH
7.4 ±0.2 at 25 ˚C (26.50 g/ l)
Contents
Ingredients
Gram/Litre
Peptic digest of animal tissue
7.000
Yeast extract
3.000
Bile salts
1.500
Lactose
10.000
Sodium chloride
5.000
Neutral red
0.030
Crystal violet
0.002
pH at 25˚C
7.4 ±0.2
Directions
Dissolved 26.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and DO NOT AUTOCLAVE. Cool to 45°C and dispense into sterile tubes containing
the inoculum.
