For the isolation and enumeration of soil microorganisms like Rhizobium species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light Beige colored
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Whitish brown colored opalescent gel
pH
6.8 ±0.2 at 25˚C (2 7.80 g/ l)
Solidification
40˚C
Contents
Ingredients
Gram/Litre
Yeast extract
1.000
Mannitol
10.000
Dipotassium phosphate
0.500
Magnesium sulphate
0.200
Sodium chloride
0.100
Calcium Carbonate
1.000
Agar
15.000
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 27.80 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
