Yeast Mannitol Broth

ARTICLE NO. OLFC-1995
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For cultivation of Rhizobium species.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Beige colored powder
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Buff white colored opalescent
pH 6.8 ±0.2 at 25˚C (12.80 g/ l)

Contents

Ingredients Gram/Litre
Yeast extract1.000
Mannitol10.000
Dipotassium phosphate0.500
Magnesium sulphate0.200
Sodium chloride0.100
Calcium carbonate1.000
pH at 25˚C6.8 ±0.2


Directions

Dissolve 12.80 grams in 1000 ml distilled water, boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.