For the cold enrichment and recovery of Yersinia enterocolitica and Yersinia
pseudotuberculosis from foods.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Pinkish beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Pink colored opalescent solution
pH
7.9 ±0.2 at 25 ˚C (26.50 g/ l)
Contents
Ingredients
Gram/Litre
Yeast Extract
5.000
Disodium Phosphate
17.250
Bile Salts
2.000
Sodium Chloride
1.000
Magnesium Sulphate
0.010
Sodium Pyruvate
1.000
Rose Bengal
0.040
pH at 25˚C
7.9 ±0.2
Directions
Dissolve 26.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to room
temperature and inoculate test sample aseptically.
Note: For more selectivity dissolve 26.50 grams in 900 ml distilled water. Boil to dissolve the
medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to
room temperature and add 100 ml of 4% filter sterilized sorbose solution. Mix well and dispense
aseptically as desired.
