For the maintenance and cultivation of Actinomyces species
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored slightly opalescent solution with a slight precipitate.
pH
7.2 ±0.2 at 25˚C (57.22 g/ l)
Contents
Ingredients
Gram/Litre
Beef Heart Infusion
10.000
Tryptose
10.000
Casein Enzymic Hydrolysate
4.000
Yeast Extract
5.000
Dextrose
5.000
L-Cysteine Hydrochloride
1.000
Starch Soluble
1.000
Sodium Chloride
5.000
Monopotassium Phosphate
15.000
Ammonium Sulphate
1.000
Magnesium Sulphate
0.200
Calcium Chloride
0.020
pH at 25⁰C
7.2 ±0.2
Directions
Dissolve 57.22 grams in 1000 ml distilled water, boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C
and inoculate test sample aseptically.
