For cultivation and maintenance of the anaerobic Actinomyces species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent solution
pH
6.9 ±0.2 at 25℃ (77.22 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Special infusion solids#
10.000
Tryptase
10.000
Tryptone
4.000
Yeast extract
5.000
Dextrose
5.000
L-Cysteine
1.000
Starch, soluble
1.000
Starch, soluble
5.000
Potassium phosphate
15.000
Ammonium sulphate
1.000
Magnesium sulphate
0.200
Calcium chloride
0.200
Agar
20.000
pH at 25 ˚C
6.9 ±0.2
Directions
Dissolve 77.22 grams in 1000 ml distilled water, boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and
distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
