For maintenance of mannitol positive Acetobacter species
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Off white to beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Milky white colored opalescent solution with slight precipitation
pH
7.4±0.2 at 25℃ (23.00 g/ l)
Color
Light yellow colored opalescent solution
pH
7.4±0.2 at 25 ˚C (48.00 g/ l)
Solidification
40˚C
Color
Light yellow colored opalescent gel
Contents
Ingredients
Gram/Litre
Yeast Extract
5.00
Peptone
3.00
Mannitol
25.00
Agar
15.00
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 48.00 grams in 1000 ml distilled water, boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
