Milky white colored opalescent solution with slight precipitation
pH
7.4±0.2 at 25℃ (23.00 g/ l)
Contents
Ingredients
Gram/Litre
Yeast Extract
10.00
Calcium Carbonate
10.00
Dextrose
3.00
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 29.18 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in test tubes. Slant the tubes to have a small butt and slant, allow to solidify. Ensure complete solidification and inoculate test sample aseptically.
