For the differentiation of Shigella species from those of the Escherichia genus.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Greenish beige colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Forest green colored slightly opalescent
pH
6.7±0.2 at 25 ˚C (29.18 g/ l)
Solidification
40˚C
Color
Forest green colored slightly opalescent gel
Contents
Ingredients
Gram/Litre
Sodium acetate
2.000
Magnesium sulphate
0.100
Sodium chloride
5.000
Monoammonium phosphate
1.000
Dipotassium hydrogen phosphate
1.000
Bromothymol Blue
0.080
Agar
20.000
pH at 25˚C
6.7±0.2
Directions
Dissolve 29.18 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in test tubes. Slant the tubes to have a small butt and slant, allow to solidify. Ensure complete solidification and inoculate test sample aseptically.
