For isolation, enumeration and differentiation of members of Enterobacteriaceae from pharma,
dairy & food products
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
orange pink colored, clear opalescent solution with fine precipitate
Solubility
Soluble in Distilled/de-ionized water at 50°C
pH
7.50±0.2 at 25˚C (41.50 g/ l)
Solidification
40 ˚C
Contents
Ingredients
Gram/Litre
Peptone
10.000
Lactose
10.000
Dipotassium hydrogen phosphate
2.000
Eosin Y
0.400
Methylene Blue
0.065
Agar
15.000
pH at 25˚C
7.1 ±0.2
Directions
Dissolve 37.46 grams in 1000 ml distilled water check. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically. Ensure complete solidification and inoculate test sample aseptically.
