Mannitol Salt Agar Base

ARTICLE NO. OLFC-1090
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For selective isolation of pathogenic Staphylococci from clinical and non-clinical samples


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Light pink beige colored powder
Solubility Soluble in Distilled/de-ionized water at 50°C
Color Orange to red colored opalescent solution
pH 7.40 ±0.2 at 25 ˚C (111.00 g/ l)
Solidification 40 ˚C
Color Orange to red colored slightly opalescent gel

Contents

Ingredients Gram/Litre
Pancreatic Digest of Casein5.000
Peptic Digest of Animal Tissue5.000
Meat Extract1.000
D-Mannitol10.000
Sodium Chloride75.000
Phenol Red0.025
Agar15.000
pH at 25˚C7.4 ±0.2


Directions

Dissolve 111.00 grams in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C, and distribute in sterile petri plates and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.