For selective isolation and enumeration of faecal Streptococci in surface water by direct plating or
by membrane filter technique.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light greenish beige colored powder
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light purple to colored opalescent
pH
7.2 ±0.2 at 25 ˚C (76.40 g/ l)
Solidification
40 ˚C
Color
Light purple colored opalescent gel
Contents
Ingredients
Gram/Litre
Proteose Peptone
10.000
Yeast Extract
10.000
Sodium Chloride
5.000
Sodium Glycerophosphate
10.000
Maltose
20.000
Lactose
1.000
Sodium Azide
0.400
Brompcresol Pruple
0.015
Agar
20.000
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 76.4 grams in 1000 ml distilled water. Boil to dissolve the medium completely and additionaly heat for 5 minutes. Avoid overheating and DO NOT AUTOCLAVE. Add 10 ml of TTC solution 1% to the medium at 50°C and mix well, distribute aseptically in petri plates for membrane filter procedure (if pour plate technique is used, kept the medium at 45°C). Ensure complete solidification and inoculate test sample aseptically.
