For differential isolation of gram-negative enteric bacteria from clinical and non-clinical samples.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Pinkish purple colored powder
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Purple with light greenish sheen colored opalescent
pH
7.2 ±0.2 at 25 ˚C (36.00 g/ l)
Solidification
40 ˚C
Color
Purple with light greenish sheen colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
10.000
Lactose
5.000
Sucrose
5.000
Dipotassium Phosphate
2.000
Eosin Y
0.400
Methylene Blue
0.065
Agar
13.500
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 36.00 grams in 1000 ml distilled water check. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically. Ensure complete solidification and inoculate test sample aseptically.
