For selective isolation and enumeration of yeasts and moulds from food samples
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Pinkish colored powder
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Pink colored clear to slightly colored opalescent
pH
5.6 ±0.2 at 25 ˚C (31.50 g/ l)
Solidification
40 ˚C
Color
Pink colored clear to slightly colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
5.000
Dextrose
10.000
Potassium Phosphate
1.000
Magnesium sulphate
0.500
Rose Bengal
0.025
Chloramphenicol
0.100
Dichloran
0.002
Agar
15.000
pH at 25˚C
5.6 ±0.2
Directions
Dissolve 31.50 grams in 1000 ml distilled water check. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
