For determination of faecal coliforms in water and foods by MPN technique.
Specifications
PHYSICAL PARAMETERS
Description
A fine free flowing, hygroscopic powder
Color
Light beige coloured powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber coloured opalescent solution
pH
6.9 ± 0.2 at 25°C (31.50 g/l)
Color
Light beige coloured powder
Contents
Ingredients
Gram/Litre
Tryptone
20.00
Lactose
5.00
Sodium Chloride
5.00
Salicin
0.50
Triton-X100
1.00
pH at 25℃
6.9 ±0.2
Directions
Dissolve 31.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and dispense into tubes containing inverted Durham tube without bubble. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45°C and inoculate test sample aseptically.