A-1 Broth (A-1 Medium)

ARTICLE NO. OLFC-100
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For determination of faecal coliforms in water and foods by MPN technique.


Specifications

PHYSICAL PARAMETERS
Description A fine free flowing, hygroscopic powder
Color Light beige coloured powder.
Solubility Soluble in Distilled/de-ionized water at 50°C
Color Light amber coloured opalescent solution
pH 6.9 ± 0.2 at 25°C (31.50 g/l)
Color Light beige coloured powder

Contents

Ingredients Gram/Litre
Tryptone20.00
Lactose5.00
Sodium Chloride5.00
Salicin0.50
Triton-X1001.00
pH at 25℃6.9 ±0.2


Directions

Dissolve 31.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and dispense into tubes containing inverted Durham tube without bubble. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45°C and inoculate test sample aseptically.